The inoue method for preparation and transformation of competent e. Coli: "ultra-competent" cells.
نویسندگان
چکیده
DMSO Inoue transformation buffer (please see Step 1) Chilled to 0°C before use. Plasmid DNA (recombinant plasmid) Construct using one of the methods described in Directional Cloning into Plasmid Vectors, Attaching Adaptors to Protruding Termini, Blunt-ended Cloning into Plasmid Vectors, Dephosphorylation of Plasmid DNA, Addition of Synthetic Linkers to Blunt-ended DNAand Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose.
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ورودعنوان ژورنال:
- CSH protocols
دوره 2006 1 شماره
صفحات -
تاریخ انتشار 2006